IN VITRO FERTILISATION
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What is In Vitro fertilisation?
In Vitro fertilisation (IVF) is an assisted reproduction treatment where the eggs are fertilized with sperm in the laboratory.
What are the steps of the process?
In Vitro fertilisation (IVF) consists of 3 main steps:
Ovarian stimulation + gynaecological tests
egg collection + sperm sample
In Vitro fertilisation and embryo culture
Embryo transfer
Cyprus Fertility Centre
How is the treatment performed?
General information
Who should have IVF Treatment ?
Couples who have unprotected intercourse for more than a year but who cannot conceive and couples who live in the following situations may need to have IVF treatment.
- Women whose fallopian tubes have been surgically removed
- Women with blocked fallopian tubes
- Women with hormonal disorders
- Women with endometriosis and chocolate cysts (also known as ovarian endometriomas)
- Women diagnosed with polycystic ovarian syndrome (PCOS)
- Men with low sperm count (oligospermia)
- Men whose sperm motility is lower than normal (asthenozoospermia or asthenospermia)
- Men who are unable to conceive naturally because of poor sperm morphology (teratozoospermia)
- Couples with un-diagnosed infertility or secondary infertility (unable to conceive second child)
Which previous tests are necessary?
To perform In Vitro fertilisation (IVF), previous examination with the following diagnostic tests is necessary:
- Hormone test to examine the ovarian reserve. The Anti-Müllerian Hormone (AMH) value can be determined at any time during the cycle, and the FSH and Estradiol value on day 3 of the cycle.
- Semen analysis to evaluate the quality of the sample. If sperm quality is very low, it will be necessary to extend the andrological examination with other additional tests.
- Gynaecological ultrasound to rule out uterine and ovarian diseases. It is also used to evaluate the ovarian reserve by antral follicle count.
- Karyotypes to rule out genetic abnormalities which may affect the embryo.
- Serologic test to dismiss the presence of transmissible infectious diseases.
Can I resume normal life during In Vitro fertilization?
It is recommended to lead a quiet life during treatment, avoiding significant strain and sports. On the day of the egg puncture, you must rest at home. You should also avoid having sexual intercourse throughout the process.
How often can In Vitro fertilisation be performed and what is the attempt limit?
Ideally you should wait until the ovaries return to their normal condition before stimulating them again for In Vitro fertilisation. It is recommended to wait a couple of cycles before performing another treatment.
There is no limit to the number of in Vitro fertilisations (IVF) but it is usually not more than 3. It is convenient to re-evaluate the case after each cycle which did not achieve a pregnancy, in order to determine whether more tests are necessary
What are the possible risks linked to the treatment?
The most important risks of In Vitro fertilisation (IVF) originates from:
Ovarian stimulation:
Hyperstimulation syndrome may occur due to the excessive response of the ovaries to the medication. This risk is currently minimal since individualised protocols are performed.
Ovarian puncture:
Bleeding can occur, which is generally self-limited. In rare occasions surgery will be required to solve this.
Multiple pregnancy
The single embryo transfer policy is helping to considerably reduce considerably the number of multiple pregnancies.
Ovarian stimulation and gynaecological tests
How are the ovaries stimulated?
To perform In Vitro fertilisation (IVF) with good chances of success, a sufficient number of eggs need to be collected. This will be achieved by subjecting the woman to controlled ovarian stimulation through the administration of subcutaneous hormone injections. The stimulation regimen will be individually adjusted according to the woman’s ovarian reserve. During stimulation, ovarian response should be monitored by serial ultrasounds until there are the correct number of appropriately sized follicles. At this point, ovulation will be triggered for collecting the eggs 36 hours later.
How often do I have to go to the clinic during ovarian stimulation?
In the majority of cases, no more than three ultrasound scans will be required to schedule the egg collection.
Is it possible to do an in IVF without stimulation?
In Vitro fertilisation within a natural cycle is indicated in cases of women with previous treatments and very low response, who seek the collection of a single egg that has been naturally selected. This option has low success rates and is reserved for cases where the patient does not accept egg donation.
Egg and sperm collection, fertilisation
How are the eggs collected?
The eggs are collected through ovarian puncture guided by ultrasound. It is a simple procedure that lasts for around 10 minutes and will be performed under sedation. It is not a painful technique as the woman will be asleep throughout the entire procedure. Since it is an outpatient process, the patient will be able to go home a few hours after egg collection.
What type of sperm can be used in Vitro fertilisation?
The sperm used for In Vitro fertilisation can come from the partner’s sperm sample or donor sperm.
In the event of using donor sperm, it will be chosen as set out by Law, maintaining the maximum phenotypic similarity with the patients.
How is the sperm collected?
The most common way of collecting sperm is through masturbation on the day of egg collection. Where necessary, a previously frozen sperm sample can be used.
Other ways of collecting sperm are testicular aspiration (TESA) and testicular biopsy (TESE). Both techniques are used when no spermatozoa are found in the ejaculate.
In case donor sperm is used, the sample will always be frozen.
How is egg fertilisation performed?
Fertilisation is performed in the laboratory after egg collection. The laboratory techniques for inseminating eggs are conventional IVF, intracytoplasmic sperm injection (ICSI) or intracytoplasmic morphologically selected sperm injection (IMSI).
ICSI is currently the most common technique of choice to inseminate eggs in the majority of cases, especially if there has been a history of fertilisation failure or very poor sperm quality.
We recommend using IMSI when there is severe teratospermia or high sperm DNA fragmentation.
For the remaining cases, if we have a good number of mature eggs, conventional IVF can be used.
What happens in the laboratory between egg fertilisation and their transfer?
18-21 hours after inseminating the eggs, its correct fertilisation is checked. The embryos will remain inside an incubator, which provides the ideal conditions for their development. These incubators can be conventional or with time-lapse technology.
The progress of each embryo will be examined every day until day 5 of development when its transfer inside the uterus will take place. This long culture of 5 days allows us to select those embryos (blastocysts) with the best implantation capacity, thereby increasing the chances for a pregnancy.
Embryo transfer
How are the embryos transferred?
Embryo transfer is a simple procedure which does not require a hospital admission or anaesthesia. A catheter is inserted into the cervical canal to place the embryos inside the uterus. Usually 1-2 embryos are transferred, although the current trend is to transfer a single embryo in order to reduce the risks of a multiple pregnancy.
Can I do something to improve my chances after the transfer?
It is very common to wonder what you can do after the transfer to encourage a pregnancy. There is nothing else you can do to increase the chances for a pregnancy, apart from wait for the embryos to implant correctly and resume a normal and relaxed lifestyle.
What should be done after embryo transfer?
After the transfer, the progesterone therapy that was started after egg collection should be continued. If all goes well, the embryo will have implanted a few of hours after the transfer, even though we will not know until the day of the pregnancy test.
What happens to the embryos that are not transferred?
If there are still some good quality embryos after embryo transfer, they will be frozen.
The transfer of frozen embryos just requires good endometrial development by the woman. Therefore, an oral or transdermal hormone therapy is used which is administered from the start of the cycle. A few days later, we check that the endometrium has been prepared correctly and the transfer day is arranged.
The embryos are thawed on the day of their transfer. If the frozen embryos are of good quality and, even more if they are blastocysts, their survival rate is higher than 90%.
The embryos can stay frozen until their final destination established by law. is decided. The freezing time does not affect the results.
Positive results
In Vitro fertilisation (IVF) is one of the best assisted reproduction treatments available. Its success rates depend mainly on the woman’s age, with the cumulative rate of pregnancy per cycle of more than 50% until the age of 39 and gradually decreasing from then on.
Women aged < 35
Aged between 18 and 35
Aged between 39 and 42
Women aged < 42
